by Cristina Colangelo, Alberto Muñoz, Alberto Antonietti, Vishal Sood, Alejandro Antón-Fernández, Joni Herttuainen, Armando Romani, Javier DeFelipe, Srikanth RamaswamyThe hindlimb representation in the somatosensory cortex of two-week old Wistar rats has been a valuable model system for dissecting the microcircuitry of neurons and their synaptic connections. In this study, we present a comprehensive experimental dataset quantifying the fiber length per cortical volume and the density of varicosities for cholinergic, catecholaminergic, and serotonergic neuromodulatory systems within the cortical neuropil using immunocytochemical staining and stereological techniques, along with a methodological framework for generating biophysically detailed computational models from these data. Acquired data were integrated into a biophysically detailed computational model of the somatosensory cortex to explore the anatomical organization and functional implications of neuromodulatory innervation. We found that neuromodulatory innervation, although sparse, substantially impacts network activity. Network simulations support the hypothesis that acetylcholine suppresses slow oscillations and promotes the desynchronization of cortical networks, consistent with the extensive findings in existing literature. Additionally, the temporal properties of acetylcholine modulation are consistent with synaptic rather than volume release. Furthermore, we found that the release of dopamine and serotonin in sensory cortices induces network desynchronization by inhibiting delta oscillations and that serotonin also initiates the emergence of theta oscillations, pointing to previously unexplored aspects of their function in governing cortical network activity. The experimental data and the biophysical computational model are available as an open-access community resource.