US-listed spot Bitcoin ETFs saw their biggest one-day outflow since June on Wednesday, as investors withdrew almost $490 million amid Bitcoin (BTC) falling to $82,000 and reversing all of the funds’ gains made in early October to make the monthly flows negative.Largest one-day outflow since late JuneSoSoValue data reported a single-day net outflow amounting to $487 million. The volume is bigger than the previous record of the year made back on June 25, when funds redeemed $691.7 million.Various sources estimated the single-day outflow to be around 2.1 standard deviations below the 90-day average daily flow, amounting to about $92 million inflow. In a standard distribution, the values lie within two standard deviations in 95 percent of cases.To give you an idea of how much the sentiment has changed, reports show that back in March, the biggest one-day outflow in three weeks was $171.12 million while Bitcoin traded close to $70,000.BlackRock’s IBIT led the redemptionsThe selling took place largely in the largest fund. The iShares Bitcoin Trust by BlackRock (IBIT) saw $207.7 million in outflows. This made up about 43% of all the funds sold on the day.FBTC by Fidelity saw a loss of $105.1 million, and ARKB of ARK 21Shares saw losses totaling $101.7 million, for a massive $206.8 million in outflows from both funds combined. GBTC by Grayscale lost $39.3 million while the BITB by Bitwise lost $27.6 million in outflows.The funds saw an inflow of $118.8 million just one session earlier, with a total of $122 million going into IBIT. Bitfinex analysts earlier in the week pointed out that the ETF inflows have already fallen about 90% from last week, with fresh spot buying necessary to make a new attempt at $90,000.October’s early gains vanished in a dayWith four trading sessions gone by in October, there were already inflows of $321.6 million into the ETFs: $102.7 million on Oct. 1, $189.9 million on Oct. 2, an outflow of $89.8 million on Oct. 5, and an inflow of $118.8 million on Oct. 6. The Wednesday outflow wiped out most of that, putting the month to a negative $163.3 million by Oct. 7. CoinDesk’s estimate for October showed a similar outflow of $165.6 million.This brings year-to-date net inflows down to just $717 million, considering the low cushion since the cumulative net flows dropped to a negative $5.76 billion in July. Since the launch of the funds in January 2024, they have managed a net inflow of $57.33 billion.Bitcoin tests the floor that has held since SeptemberBitcoin price dropped by more than 3% over the last 24 hours. BTC is trading at an average price of $80,642 at the time of press. This comes following a close at $85,771 on October 5, $85,540 on October 6, and $83,282 on October 7, marking a test of the price floor established since the rally lost traction on September 21.Glassnode gave a more tempered take, suggesting combined spot-exchange and ETF volume has averaged about $6.8 billion a day for seven days, lighter than nine out of ten periods since January 2024. Glassnode located the closest liquidation zone between $81,700 and $83,300, precisely where Bitcoin is trading currently, with a block of Binance spot bids at $81,000 and $81,250.Ether funds are bleeding tooIt was not only Bitcoin that pulled back recently. The outflow of U.S. spot Ether ETFs reached another $160.9 million on Oct. 7, marking the seventh consecutive day of declines from Sept. 29. ETHA of BlackRock saw an outflow of $116.1 million, while Grayscale’s ETHE experienced outflows of $25.8 million. Ether ETF funds lost around $568.8 million in total in seven negative periods.There was one indicator moving in the opposite direction. Santiment recorded a net withdrawal of 24,073 BTC from exchanges on Oct. 5, the highest one-day outflow in seven months, reducing exchange reserves to just below 6.5% of the total supply. This is seen as positive by the firm since there are fewer Bitcoins that could be sold, although outflows alone do not guarantee price hikes.The smartest crypto minds already read our newsletter. Want in? Join them.