by Aniruddha Chattaraj, David S. Kanovich, Srivastav Ranganathan, Eugene I. ShakhnovichPhase separated condensates are recognized as a ubiquitous mechanism of spatial organization in cell biology. Biophysical modeling of condensates provides critical insights into the dynamics and functions of these subcellular structures that are difficult to extract via experiments. Here we present an efficient computational pipeline, CASPULE (Condensate Analysis of Sticker Spacer Polymers Using the LAMMPS Engine), to simulate and analyze the biological condensates made of sticker-spacer polymers. CASPULE implements a unique force field that combines traditional Langevin dynamics with a “detailed-balance proof” protocol for single-valent bond formation between stickers. This framework allows us to study the non‑trivial biophysics that emerge from single‑valent sticker interactions, coupled with the effect of separating the energetic contributions of stickers and spacers. We provide detailed documentation on how to setup the simulation environment, perform simulations and analyze the results. Through case studies, we highlight the utility and efficacy of our pipeline. Importantly, we provide statistical parameters to characterize the cluster size distribution often observed in biological systems. We envision this tool to be broadly useful in decoding the interplay of kinetics and thermodynamics underlying the formation and function of biological condensates.