Reference materials should be adopted as a common calibrator for multiomics measurement and co‑profiled with study samples. Multiomics results should be reported as sample‑to‑reference ratios so that they are reproducible and suitable for artificial intelligence tools.This is a preview of subscription content, access via your institutionAccess options Access through your institutionAccess Nature and 54 other Nature Portfolio journalsGet Nature+, our best-value online-access subscription27,99 € / 30 dayscancel any timeLearn moreSubscribe to this journalReceive 12 print issues and online access269,00 € per yearonly 22,42 € per issueLearn moreBuy this articlePurchase on SpringerLinkInstant access to the full article PDF.39,95 €Prices may be subject to local taxes which are calculated during checkoutFig. 1: Root cause of multiomics data irreproducibility and the role of reference materials as a common calibrator.SubjectsMetabolomicsProteomicsResearch dataTranscriptomicsReferencesRoohani, Y. H. et al. Cell 188, 3370–3374 (2025).Article CAS PubMed Google Scholar Wang, X. et al. Nat. Rev. Clin. 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Biotechnol. 42, 1118–1132 (2024).Article CAS PubMed Google Scholar Download referencesAcknowledgementsWe thank F. Goodsaid, Z. Peng and W. Xu for comments on early versions of the manuscript. L.S. and Y.Z. were supported in part by the National Key R&D Project of China (2023YFC3402500 and 2023YFC3402501). The views expressed are those of the authors and do not necessarily represent the views or policies of their respective organizations.Author informationAuthors and AffiliationsFudan University and International Human Phenome Institutes, Shanghai, ChinaLeming Shi, Ying Yu & Yuanting ZhengJMP Statistical Discovery, SAS Institute, Cary, NC, USAWenjun Bao & Russell D. WolfingerUniversity of Limerick, Limerick, IrelandAedín C. CulhaneNational Institute of Metrology, Beijing, ChinaXiang FangHK3 Lab S.R.L, Orobix Life & LIGHT Center Brescia, Brescia, ItalyCesare FurlanelloopAIda, Inc, Princeton, NJ, USARuixin HaoIQVIA Laboratories, Durham, NC, USAWendell D. JonesCellino Biotech, Inc, Cambridge, MA, USARebecca KuskoOffice of the Commissioner, US Food and Drug Administration, Silver Spring, MD, USASamir LababidiWeill Cornell Medicine, New York, NY, USAChristopher E. MasonInstitute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, FinlandAndreas SchererCenter for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, USAShraddha ThakkarNational Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USAWeida Tong & Joshua XuNational Center for Clinical Laboratories, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaRui ZhangAuthorsLeming ShiView author publicationsSearch author on:PubMed Google ScholarWenjun BaoView author publicationsSearch author on:PubMed Google ScholarAedín C. CulhaneView author publicationsSearch author on:PubMed Google ScholarXiang FangView author publicationsSearch author on:PubMed Google ScholarCesare FurlanelloView author publicationsSearch author on:PubMed Google ScholarRuixin HaoView author publicationsSearch author on:PubMed Google ScholarWendell D. JonesView author publicationsSearch author on:PubMed Google ScholarRebecca KuskoView author publicationsSearch author on:PubMed Google ScholarSamir LababidiView author publicationsSearch author on:PubMed Google ScholarChristopher E. MasonView author publicationsSearch author on:PubMed Google ScholarAndreas SchererView author publicationsSearch author on:PubMed Google ScholarShraddha ThakkarView author publicationsSearch author on:PubMed Google ScholarWeida TongView author publicationsSearch author on:PubMed Google ScholarRussell D. WolfingerView author publicationsSearch author on:PubMed Google ScholarJoshua XuView author publicationsSearch author on:PubMed Google ScholarYing YuView author publicationsSearch author on:PubMed Google ScholarRui ZhangView author publicationsSearch author on:PubMed Google ScholarYuanting ZhengView author publicationsSearch author on:PubMed Google ScholarContributionsL.S. and Y.Z. drafted the manuscript. All authors contributed to the development of the concepts, reviewed the manuscript and approved its submission.Corresponding authorsCorrespondence to Leming Shi or Yuanting Zheng.Ethics declarationsCompeting interestsCertain authors are employees of SAS Institute, IQVIA, Cellino Bio, FDA, and Weill Cornell Medicine. L.S. and Y.Z. are co-leaders of the MAQC Quartet and Plasmix projects.Rights and permissionsReprints and permissionsAbout this article