Thousands of rallies are taking place across the United States on Saturday under the banner of the ‘No Kings’ protest movement, the third nationwide demonstration led by the group that has become a national symbol of opposition to President Donald Trump. Organizers are anticipating what could be the largest day of domestic political protest in U.S. history, with more than 3,300 events planned in all 50 states. The flagship protest is taking place in Minneapolis—St Paul, Minnesota, the site of an aggressive immigration crackdown by the Trump Administration that led to the killing of Renee Good and Alex Pretti by federal agents. Read more: 'No Kings' Protests May Draw Biggest—and Most Diverse—Anti-Trump Crowds EverU.S. Sen. Bernie Sanders, Governor Tim Walz, Lieut. Governor Peggy Flanagan, Attorney General Keith Ellison, and U.S. Rep. Ilhan Omar are set to speak at the rally in the Twin Cities. Bruce Springsteen is set to perform at the rally alongside Joan Baez, Maggie Rogers, Jane Fonda and other musicians. “From illegal wars to masked agents in our streets to driving up costs and trying to control our elections before they happen, this administration’s actions are not just angering deep blue urban centers, but they are crossing a red line for everyday families in the suburbs, too,” organizers said in a statement on the No Kings website on Friday. The two previous No Kings protests drew millions of participants across the country in protest against what organizers viewed as Trump’s rising authoritarianism and corruption. Approximately 5 million people took to the streets in the first “No Kings” protest last June. In October, an estimated 7 million people took part. Those first demonstrations were motivated by Trump’s unprecedented use of presidential power in his first year at home. By October, Trump had ordered the National Guard into Democratic-run cities to quell protests and aid in immigration enforcement, launched a crackdown on left-wing and liberal groups, and implemented a sweeping mass deportation program that has seen masked federal agents conducting military-style raids and detaining people without due process.Since then, Trump has launched a deeply unpopular war with Iran alongside Israel that has been raging for one month and has had far-reaching negative impacts on the global economy, as well as killing more than 1,500 civilians in Iran and 13 U.S. service members. The Department of Homeland Security, meanwhile, has been shut down since Feb. 14 amid a standoff between Democrats and Republicans over immigration enforcement, leading to hours-long security lines at airports that are struggling with a staffing shortage among Transportation Security Administration agents.Polling in recent days has found Trump’s approval ratings sinking to their lowest levels since he returned to the White House.Here are the cities most likely to host the largest protests. Minneapolis—Saint PaulThe Twin Cities are the flagship site of the “No Kings” protests. In January, federal officers fatally shot two American citizens—Renee Good and Alex Pretti—in two separate incidents in Minneapolis. The shootings sparked massive protests, with thousands of people taking to the city’s streets.The event on Saturday is scheduled to start at noon CST at three locations in Saint Paul, then participants march to the Minnesota State Capitol for a rally in the afternoon. Bruce Springsteen is set to perform, and several other celebrities—including Joan Baez, Jane Fonda, and Maggie Rogers—are expected to be in attendance. Independent Sen. Bernie Sanders of Vermont is also expected to make an appearance.“This is the main event,” event organizers said in an Instagram post about the Twin Cities protests. “The one the whole country is watching.”“This isn’t just another local protest,” they continued. “It’s a nationwide spotlight. If you’re in Minnesota, you’re not on the sidelines. You’re at the center of it. This is going to be massive. We’ll see you there.”Washington, D.C.Several events are being held in the Washington, D.C., area this Saturday, including a march into the city set to begin at 10 a.m. ET and rally on the National Mall starting at 1 p.m. ET. The previous “No Kings” demonstrations in October saw more than 200,000 protesters in the nation’s capital, organizers estimated, so it’s likely that this weekend’s protest will also draw a massive crowd.ChicagoOrganizers have planned multiple demonstrations in the Chicago area this Saturday.“On March 28th, we will rise up, take to the streets, and say it loud: no thrones, no crowns, no kings—Hands off Chicago and hands off our democracy,” reads the description for one of the events, scheduled to begin at 1:30 p.m. CT in the city’s Grant Park. “As the atrocities from the Trump regime have grown, so has our resistance in Chicago and across the country. Help us show the nation and the world that Chicago is united in opposing the illegal, cruel, and destructive attacks against our neighbors, our communities and our democracy.”In October, roughly 250,000 protesters took to the streets of Chicago, organizers estimated.New York CityIn New York City, several protests are set to take place across multiple boroughs this Saturday, including a march that will start at Central Park South in Manhattan at 2 p.m. ET.More than 100,000 protesters participated in the October demonstrations across all five boroughs, according to the New York Police Department. Some estimates from organizers put the number significantly higher, at more than 350,000 people.The San Francisco-Bay AreaThe Bay Area has organized a number of events for the day of action, including in San Francisco, Berkeley, and Oakland. Among them, a San Francisco march is set to begin in the city’s Embarcadero Plaza at 11:30 a.m. PT.In October, as many as 220,000 demonstrators participated in the “No Kings” movement in the Bay Area, according to a survey of organizers conducted by the Bay Area News Group.Additional reporting by Chantelle Lee and Thiago Ventura.