by Marine A. Courtois, Louise van Oudenhove, Suzanne Touzeau, Frédéric Grognard, Ludovic MailleretThe sterile insect technique (SIT) is a pest control strategy based on the mass release of sterilized males to disrupt natural reproduction and suppress wild populations. However, its effectiveness can be challenged by biological factors such as female multiple mating and sperm use bias. While multiple mating is widespread among many insect species, the mechanisms governing sperm use remain poorly understood. In this study, we develop and analyze a compartmental mathematical model based on differential equations to investigate the overall impact of multiple mating on SIT efficiency. We further analyze the effect of sperm use biases with an agent-based model, calibrated on Drosophila suzukii, allowing the exploration of different scenarios: preferential use of first vs last sperm, of fertile vs sterile sperm, and mixed sperm use. Our results highlight how multiple mating and sperm use biases influence SIT effectiveness. In the longer term, multiple mating is disadvantageous as it requires additional releases of sterilized males to control the pest population. However, in the shorter term, it can be beneficial by disrupting further female reproductive output by “defertilizing” females mated with wild males. This study provides new information on how the way sperm is processed after mating can impact sterile insect control strategies, highlighting the limited influence of these biological processes depending on the release efforts that can be deployed.