Following the rise in Mpox cases, particularly in countries where the disease had not traditionally been observed, the World Health Organization (WHO) declared a Public Health Emergency of International Concern in June 2022 and again in August 2024. Accurate and timely diagnosis plays a critical role in controlling the infection. However, PCR-based methods—the gold standard for Mpox diagnosis—require complex laboratory infrastructure and trained personnel, making them less accessible in many settings. For this reason, the development of point-of-care diagnostic tools is of great importance.