Knowledge, attitudes, and practices (KAP) studies help identify priority groups for control interventions. They include multiple factors, such as threat perception (encompassing perceived susceptibility and severity of a health risk) and sociodemographic data, which have been shown to be associated with individual prevention and control measures for mosquito-borne diseases. However, their application to tick-borne diseases (TBDs) remains largely unstudied in limited-resource settings. Ehrlichia canis, transmitted by Rhipicephalus sanguineus s.l. ticks, is highly prevalent in Iquitos, Peru, a city in the Peruvian Amazon. We analyzed data from a questionnaire completed by 285 dog owners in Iquitos to assess whether the perceived threat from ticks and TBDs is linked to preventive measures for homes and dogs. Secondary aims included examining the associations between TBD awareness and preventive/control measures, as well as identifying sociodemographic factors linked to these measures and threat perception. We used simple and multiple regression models to evaluate the associations of interest. We found that 31.6% of participants (n = 90) were unaware that TBDs could occur in humans. This group tended to be older, with lower education levels and lower income. Only a small portion of the included participants reported using preventive/control measures. After adjusting for sociodemographic factors, TBD awareness was associated with a higher likelihood of home spraying. Furthermore, those who perceived TBDs as moderate diseases were marginally more likely to apply spray acaricide to their dogs, while those who perceived these diseases as severe to very severe were more likely to administer oral acaricides to their dogs. These findings highlight variability in the link between threat perception and preventive measures. Results suggest TBD awareness campaigns may benefit from focusing on older individuals with lower education and income, while educational efforts promoting effective prevention measures like acaricidal pills should target all dog owners.