A research team led by Associate Professor Hirofumi Nishizono and graduate student Masaki Kato from the Research Support Center at the Medical Research Institute of Kanazawa Medical University has identified eleven novel factors essential for the development of fertilized eggs. This achievement was made possible through the integration of one-cell embryo cryopreservation technology, an inhibitor library screening, RNA-seq analysis, and CRISPR-Cas9-mediated gene editing.